RHD*03.03 - RHD*DIIIc
(ISBT table: RHD partial D v5.0)
This entry is an hybrid RHD allele.
RHD(L121M,V127A,D128G,N152T), RHD*361A,380C,383G,455C, RHD*361A,380C,383G,455C (DIIIc), RHD*361T>A,380T>C,383A>G,455A>C,
Molecular data
Phenotype
Main D phenotype: weak D (last update: May 4, 2020)Reports by D phenotype
Other RH phenotypes: RH:-3, 12, -30, -54,
Serology with monoclonal anti-D
- 0 monoclonal IgG anti-D non-reactive with variant, out of approx 20 monoclonal IgG tested
(Table 3; control sample, probably same sample as in
10753853 ) - 0 monoclonal IgG anti-D non-reactive with variant, out of 37 monoclonal IgG and 3 IgM tested; all epitopes detected; D-epitope pattern
- cross-testing table between different D categories (Table 1, Table 2)
Antigen Density (Ag/RBC)
More phenotype data
Haplotype
Main CcEe phenotype association: Ce (last update: Jan. 8, 2021)ce | Ce | cE | CE | |
---|---|---|---|---|
ce | 0 | 2 | 0 | 0 |
Ce | 6 | 0 | 0 | |
cE | 0 | 0 | ||
CE | 0 |
Reports by CcEe phenotype
Reports by allele association
Alloimmunization
Antibodies in carriers
Antibody specificity: D (RH1)
Summary: allo-anti-D (last update: Nov. 17, 2019)Detailed information
-
Wagner FF et al. Transfus Med Hemother (2014)
(RIR n°9, 43, 45)
- Ab specificity: D (RH1)
- Number (auto- or allo-):
- Number listed as allo-: 3
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT: ND
- Autologuous control: ND
- Elution: ND
- Autoadsorption: ND
- Titer: ND
- Was anti-LW excluded?: yes
- Other antibodies detected: ND
- Cross matches (with Ab and RBCs from different partial types): ND
- Transfusion history: ND
- Pregnancy history:
- Anti-D Ig history: ND, probably none
- Context: ND
- Hemolytic consequences: ND
- Comment:
von Zabern I et al. Transfusion (2013)
-
Faas BH et al. Transfusion (1996)
- Ab specificity: D (RH1)
- Number (auto- or allo-): 2
- Number listed as allo-:
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT:
- Autologuous control:
- Elution:
- Autoadsorption:
- Titer:
- Was anti-LW excluded?:
- Other antibodies detected:
- Cross matches (with Ab and RBCs from different partial types):
- Transfusion history:
- Pregnancy history:
- Anti-D Ig history:
- Context:
- Hemolytic consequences:
- Comment: donors VvdA and MA used as controls
-
Beckers EA et al. Transfusion (1996)
- Ab specificity: D (RH1)
- Number (auto- or allo-):
- Number listed as allo-: 1
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT: negative
- Autologuous control:
- Elution:
- Autoadsorption: not autoadsorbable
- Titer: 4000 (IgG)
- Was anti-LW excluded?:
- Other antibodies detected:
- Cross matches (with Ab and RBCs from different partial types): Proposita's RBCs were compatible with anti-D of two individuals with RHef00589 (samples SvS and MS were provided from Barcelona, Spain and London, UK resp.; moreoever, sample MS underwent molecular analysis). Cross-reactivities with different RBCs (Table 1, Table 2).
- Transfusion history: 3 D+ RBC units after one delivery
- Pregnancy history:
- Anti-D Ig history:
- Context:
- Hemolytic consequences: HDN in her third D+ child with positive IgG DAT, elution of anti-D, and rapidly developing jaundice, "hemoglobin level of 9.2 mmol per L (normal range, 10.0-12.0 mmol/L) and bilirubin concentration of 410 pmol per L", one exchange transfusion was necessary on day 2
- Comment:
Antibodies in D negative recipients
Alloimmunization in recipients: expected to be possible, see phenotype data
Reports
Summary: few descriptions (last update: Feb. 22, 2020)Detailed reports
- 1 sample (+5 related) D positive who produced allo-anti-D White, in the Dutch population
- 1 sample (MS) used as control, may be a phenotypic characterization referred by a UK lab from London
- 1 sample used as control referred by a Brazilian lab from Sao Paolo
- 1 sample used as control, may be a phenotypic characterization referred by a Spanish lab from Barcelona, Catalonia
- 1/163 selected variants included for the development of a genotyping assay mainly in the Dutch population (samples may have been included in other studies)
- 1 or more among 829 samples African American (in the USA population)
- 1/94 donors with discreapancies in D typing Southeast Brazil
- 1 hemizygote among 278 samples selected for the development of nonspecific quantitative next-generation sequencing. (non-random samples, may have been reported in other studies)
Allele or phenotype frequency
Structure mapping
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References
- Beckers EA et al. Characterization of the hybrid RHD gene leading to the partial D category IIIc phenotype. Transfusion, 1996. [Citation] [RHeference]
- Faas BH et al. Involvement of Ser103 of the Rh polypeptides in G epitope formation. Transfusion, 1996. [Citation] [RHeference]
- Maaskant-van Wijk PA et al. Genotyping of RHD by multiplex polymerase chain reaction analysis of six RHD-specific exons. Transfusion, 1998. [Citation] [RHeference]
- Avent ND et al. The rhesus blood group system: insights from recent advances in molecular biology. Transfus Med Rev, 1999. [Citation] [RHeference]
- Wagner FF et al. Weak D alleles express distinct phenotypes. Blood, 2000. [Citation] [RHeference]
- Lomas-Francis C et al. DIII Type 7 is likely the original serologically defined DIIIb. Transfusion, 2012. [Citation] [RHeference]
- von Zabern I et al. D category IV: a group of clinically relevant and phylogenetically diverse partial D. Transfusion, 2013. [Citation] [RHeference]
- Daniels G et al. Variants of RhD--current testing and clinical consequences. Br J Haematol, 2013. [Citation] [RHeference]
- Haer-Wigman L et al. RHD and RHCE variant and zygosity genotyping via multiplex ligation-dependent probe amplification. Transfusion, 2013. [Citation] [RHeference]
- Fichou Y et al. A convenient qualitative and quantitative method to investigate RHD-RHCE hybrid genes. Transfusion, 2013. [Citation] [RHeference]
- Reid ME et al. Genomic analyses of RH alleles to improve transfusion therapy in patients with sickle cell disease. Blood Cells Mol Dis, 2014. [Citation] [RHeference]
- Wagner FF et al. The Rhesus Site. Transfus Med Hemother, 2014. [Citation] [RHeference]
- A C Gaspardi et al. RHD variants in blood donors from Southeast Brazil. Transfusion, 2015. — Abstract — [RHeference]
- Dezan MR et al. High frequency of variant RHD genotypes among donors and patients of mixed origin with serologic weak-D phenotype. J Clin Lab Anal, 2018. [Citation] [RHeference]
- Floch A et al. Comment from Rheference Online ressource, 2020. — Online ressource — [RHeference]
- Stef M et al. RH genotyping by nonspecific quantitative next-generation sequencing. Transfusion, 2020. [Citation] [RHeference]
Last update: Jan. 8, 2021