RHD*12.01 - RHD*DOL1
(ISBT table: RHD partial D v5.0)
This entry is an RHD allele.
DOL-1, RHD(M170T,F223V), RHD*509C,667G, RHD*509C,667G (DOL1, RHD*12.01), RHD*509T>C,667T>G,
Molecular data
Phenotype
Main D phenotype: variable/discrepant (last update: May 4, 2020)Reports by D phenotype
Other RH phenotypes: RH:-2, -3, -10, 12, -20, -23, -32, -50, 54,
- RH:-2 deduced from comparison of RH2 expression of a sample heterozygous for RHef00086 and RHef00452 and controls hemizygous RHef00452 and standard RHCE*02 inferred from the reported RHCE phenotypes of the carriers
- RH:-3 inferred from the reported RHCE phenotypes of the carriers
- RH:-10
- RH:12
- RH:-20
- RH:-23
- RH:-32
- RH:-50
- RH:54 exon 4 was not explored to distinguish DOL types
Serology with monoclonal anti-D
Antigen Density (Ag/RBC)
More phenotype data
Rhesus Similarity Index
Haplotype
Main CcEe phenotype association: ce (last update: May 4, 2020)ce | Ce | cE | CE | |
---|---|---|---|---|
ce | 4 | 0 | 0 | 0 |
Ce | 0 | 0 | 0 | |
cE | 0 | 0 | ||
CE | 0 |
Reports by CcEe phenotype
Main allele association: RHCE*01.08 (RHCE*ceBI) or RHCE*ceSMReports by allele association
Alloimmunization
Antibodies in carriers
Antibody specificity: D (RH1)
Summary: allo-anti-D (last update: Nov. 17, 2019)Detailed information
-
Flegel WA et al. Transfusion (2009)
- Ab specificity: D (RH1)
- Number (auto- or allo-):
- Number listed as allo-: 1
- Number listed as auto-:
- Number of carriers of the allele assessed: 3
- DAT: negative
- Autologuous control: ND
- Elution: ND
- Autoadsorption: ND
- Titer: ND
- Was anti-LW excluded?: yes
- Other antibodies detected: ND
- Cross matches (with Ab and RBCs from different partial types): ND
- Transfusion history: immunized by transfusion
- Pregnancy history:
- Anti-D Ig history: ND, probably none
- Context: SCD patient
- Hemolytic consequences: ND
- Comment:
von Zabern I et al. Transfusion (2013)
Wagner FF et al. Transfus Med Hemother (2014) (RIR n°27)
-
Roussel M et al. Transfusion (2013)
- Ab specificity: D (RH1)
- Number (auto- or allo-):
- Number listed as allo-: 1
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT: negative
- Autologuous control: negative
- Elution: negative
- Autoadsorption: not autoadsorbable
- Titer: 8
- Was anti-LW excluded?: yes, antibody not reactive with RH:1, LW:–5,–6,–7 (LW null) test-RBCs
- Other antibodies detected:
- Cross matches (with Ab and RBCs from different partial types): ND
- Transfusion history: probably none
- Pregnancy history:
- Anti-D Ig history: ND, probably none
- Context: pregnancy
- Hemolytic consequences: ND
- Comment:
Antibodies in D negative recipients
Alloimmunization in recipients: expected to be possible, see phenotype data
Reports
Summary: few descriptions, in Africans or possible African descent (last update: Dec. 22, 2019)Detailed reports
- 1 sample Czech of Lebanese descent
- 1 sample (1 heterozygous with RHef00452) Togolese (Ewe)
- 1 sample (1 heterozygous with RHef00447) Australian of Botswanian descent
- 1/45 (prevalence among weak D) or 1/12672 (phenotypic prevalence in population) 45 patients with weak D phenotype were genotyped in a cohort of 12672 patients from a public hospital Argentinean
- 0/43 (prevalence among weak D phenotype) or 0/5707 (phenotypic prevalence in population) 43 patients with weak D phenotype were genotyped in a cohort of 5707 patients from a private laboratory Argentinean
- 1/163 selected variants included for the development of a genotyping assay mainly in the Dutch population (samples may have been included in other studies)
- 4/351 out of 351 prenatal patients with discrepant D phenotyping results (population tested 608486 patients) Canada
- 1/23 donors with weak D phenotype (among 4458 random donors, 4028 types D positive including 19 weak D phenotype, 420 typed D negative including 4 showed to be weak D by further serological testing; in total 23 donors had weak D phenotype) in the Maroccan population
- 1 hemizygote, 3 heterozygotes among 278 samples selected for the development of nonspecific quantitative next-generation sequencing. (non-random samples, may have been reported in other studies)
Allele or phenotype frequency
Structure mapping
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References
- Flegel WA et al. D variants at the RhD vestibule in the weak D type 4 and Eurasian D clusters. Transfusion, 2009. [Citation] [RHeference]
- Brajovich ME et al. Comprehensive analysis of RHD alleles in Argentineans with variant D phenotypes. Transfusion, 2012. [Citation] [RHeference]
- Reid ME et al. The low-prevalence Rh antigen STEM (RH49) is encoded by two different RHCE*ce818T alleles that are often in cis to RHD*DOL. Transfusion, 2013. [Citation] [RHeference]
- von Zabern I et al. D category IV: a group of clinically relevant and phylogenetically diverse partial D. Transfusion, 2013. [Citation] [RHeference]
- Roussel M et al. RHD*DOL1 and RHD*DOL2 encode a partial D antigen and are in cis with the rare RHCE*ceBI allele in people of African descent. Transfusion, 2013. [Citation] [RHeference]
- Haer-Wigman L et al. RHD and RHCE variant and zygosity genotyping via multiplex ligation-dependent probe amplification. Transfusion, 2013. [Citation] [RHeference]
- Wagner FF et al. The Rhesus Site. Transfus Med Hemother, 2014. [Citation] [RHeference]
- Clarke G et al. Resolving variable maternal D typing using serology and genotyping in selected prenatal patients. Transfusion, 2016. [Citation] [RHeference]
- El Housse H et al. Comprehensive phenotypic and molecular investigation of RhD and RhCE variants in Moroccan blood donors. Blood Transfus, 2019. [Citation] [RHeference]
- Floch A et al. Comment from Rheference Online ressource, 2020. — Online ressource — [RHeference]
- Stef M et al. RH genotyping by nonspecific quantitative next-generation sequencing. Transfusion, 2020. [Citation] [RHeference]
Last update: Jan. 8, 2021