phenotypic R0Har
(ISBT table: not listed)
This entry is a phenotypic characterization.
R0Har/DHAR - phenotypic description,
Molecular data
Nucleotides:
Amino acids:
Hybrid allele encompassing at least one RHD exon:
NA
Comments on the molecular basis:
Extracellular position of one or more amino acid substitutions:
Splicing:
Unconventional prediction methods:
Phenotype
Main D phenotype: variable/discrepant (last update: Nov. 16, 2020)Reports by D phenotype
- Discrepant D phenotype (negative or positive depending on anti-D reagents and techniques)
- Reactive with 2 anti-D sera from phenotype DVI individuals
- Weak D phenotype
- Reactive with 2 anti-D sera from phenotype DVI individuals
Other RH phenotypes: RH:-2, -3, 4, 5, 6, -7, -8, -9, -10, -11, -12, 17, -18, -19, -20, -22, -23, -27, -30, -32, 33, 50, -54, 6,
- RH:-2 inferred from the reported RHCE phenotypes of the carriers
- RH:-3 inferred from the reported RHCE phenotypes of the carriers
- RH:4
- RH:5 weak
- RH:6
- RH:-7
- RH:-8
- RH:-9
- RH:-10
- RH:-11
- RH:-12
- RH:17 compatible with one of 3 immune D--/D-- sera tested; authors conclude RH:w17
- RH:-18 compatible with the Shabalala serum
- RH:-19 compatible with the Shabalala serum
- RH:-20
- RH:-22
- RH:-23
- RH:-27
- RH:-30
- RH:-32
- RH:33
- RH:50 general association, not tested in this study
- RH:-54
- RH:6 weak
Serology with monoclonal anti-D
- 7 samples tested; non-reactive with LOR-15C9 Ab
- epitope pattern (Table 1)
- tested with monoclonal anti-D; epitope pattern
- positive reaction with 4 monoclonal IgM, but only with 5 of 24 monoclonal IgG1 anti-D
- reactivity with monoclonal anti-D, tested by different laboratories (Table 1)
- reactivity pattern with 6 monoclonal anti-D
Antigen Density (Ag/RBC)
More phenotype data
Rhesus Similarity Index
Haplotype
Main CcEe phenotype association: ce (last update: Nov. 16, 2020)Alloimmunization
Antibodies in carriers
Antibody specificity: D (RH1)
Summary: allo-anti-D (last update: Nov. 16, 2020)Detailed information
Antibodies in D negative recipients
Alloimmunization in recipients: yes, see detailed reports and phenotype data
Reports
Summary: frequent descriptions, in individuals of Caucasian descent, or compatible with such descent (last update: Nov. 13, 2020)Detailed reports
- 1 sample donor German
- 1 sample (+ 2 from family study) donor British
- 30/342 out of 342 D samples sent for D phenotyping (76 were too weak for epitope determination and classification, only half of the remaining could be classified)
- >30 samples among the propositi tested by the authors White
- 2/168 among 168 samples referred for weak D phenotype and/or allo anti-D in D positive individuals, 137 were characterized in the study (70 by serology, 67 by molecular analysis, 31 could not be typed because serologic typing was inconclusive and molecular typing could not be performed) in the French population (Caucasian)
- 0/10000 screening of D positive individuals Indian (West India)
Allele or phenotype frequency
Structure mapping
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References
- Giles CM et al. An Rh gene complex which results in a "new" antigen detectable by a specific antibody, Anti-Rh 33. Vox Sang, 1971. [Citation] [RHeference]
- Issitt PD et al. Anti-Rh33, the second separable example, also made by a person who made anti-D and has C+ red cells. Transfusion, 1986. [Citation] [RHeference]
- Tippett P et al. Monoclonal antibodies against Rh and Rh related antigens. J Immunogenet, 1990. [Citation] [RHeference]
- Cartron JP et al. Tentative model for the mapping of D epitopes on the RhD polypeptide. Transfus Clin Biol, 1996. [Citation] [RHeference]
- Tippett P et al. The Rh antigen D: partial D antigens and associated low incidence antigens. Vox Sang, 1996. [Citation] [RHeference]
- Wallace M et al. The D antigen characteristic of RoHar is a partial D antigen. Vox Sang, 1996. [Citation] [RHeference]
- Jones J et al. Selection of monoclonal antibodies for the identification of D variants: ability to detect weak D and to split epD2, epD5 and epD6/7. Vox Sang, 1996. [Citation] [RHeference]
- Avent ND et al. Evidence of genetic diversity underlying Rh D-, weak D (Du), and partial D phenotypes as determined by multiplex polymerase chain reaction analysis of the RHD gene. Blood, 1997. [Citation] [RHeference]
- Reid ME et al. Use of LOR-15C9 monoclonal anti-D to differentiate erythrocytes with the partial DvI antigen from those with either partial D antigens or weak D antigens. Immunohematology, 1998. [Citation] [RHeference]
- Reid ME et al. DAK, a new low-incidence antigen in the Rh blood group system. Transfusion, 2003. [Citation] [RHeference]
- Ansart-Pirenne H et al. RhD variants in Caucasians: consequences for checking clinically relevant alleles. Transfusion, 2004. [Citation] [RHeference]
- Kulkarni S et al. Frequency of partial D in Western India. Transfus Med, 2008. [Citation] [RHeference]
- Floch A et al. Comment from Rheference Online ressource, 2020. — Online ressource — [RHeference]
Last update: Nov. 16, 2020