DIVa - phenotypic description
(ISBT table: not listed)
This entry is a phenotypic characterization.
DIVa - phenotypic description,
Molecular data
Nucleotides:
Amino acids:
Hybrid allele encompassing at least one RHCE exon:
NA
Comments on the molecular basis:
- PCR pattern, exons 3, 4, 5, 6, 7 and 9 and epitope pattern
- phenotype associated with RHef00067
- molecular typing not specified, assumed phenotypic characterization
Extracellular position of one or more amino acid substitutions:
Splicing:
Unconventional prediction methods:
Phenotype
Main D phenotype: variable/discrepant (last update: June 16, 2020)Reports by D phenotype
Other RH phenotypes: RH:-3, 12, -23, 30, -40, -50, -54,
Serology with monoclonal anti-D
- with cross-testing table between different D categories
- 6 samples tested; non-reactive with LOR-15C9 Ab
- 2 anti-D non-reactive with variant, out of 5 monoclonal anti-D tested; one polyclonal anti-D was also tested (Table 1)
- epitope pattern (Table 1)
- tested with monoclonal anti-D; epitope pattern
- differences between RHef00585 and RHef00586 (Table 5)
- 1 sample tested; positive reaction with 128 of 135 anti-D tested (Ab not detailed) (Table 1)
- 1 sample tested; positive reaction with 109 of 116 anti-D tested (Ab not detailed) (Table 1)
- 1 sample tested; positive reaction with 156 of 160 anti-D tested (Ab not detailed) (Table 1)
- reactivity with monoclonal anti-D, tested by different laboratories (Table 1)
- reactivity pattern with 6 monoclonal anti-D
Antigen Density (Ag/RBC)
- 41300 Ag/RBC, 1 sample, tested with 1 monoclonal anti-D
- 34900 Ag/RBC, 1 sample, tested with 1 monoclonal anti-D
- 31700 Ag/RBC, 1 sample, tested with 1 monoclonal anti-D
- 29300 Ag/RBC, 1 sample, tested with 1 monoclonal anti-D
- 6900 Ag/RBC, 1 sample, tested with 1 monoclonal anti-D
- range: 8000 - 9300 Ag/RBC, 2 R0r samples, tested with 5 monoclonal anti-D (H27, BRAD5, BRAD7, 2B6, BRAD3), and one polyclonal anti-D (Table 1, Figure 3)
More phenotype data
Rhesus Similarity Index
Haplotype
Main CcEe phenotype association: Ce (last update: Dec. 9, 2020)Alloimmunization
Antibodies in carriers
Antibody specificity: D (RH1)
Summary: not relevant, see types or alleles (last update: Aug. 25, 2020)Detailed information
-
Lomas C et al. Transfus Med (1993)
(Table 2)
- Ab specificity: D (RH1)
- Number (auto- or allo-):
- Number listed as allo-: 11
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT:
- Autologuous control:
- Elution:
- Autoadsorption:
- Titer:
- Was anti-LW excluded?:
- Other antibodies detected:
- Cross matches (with Ab and RBCs from different partial types): cross-matching of RBCs and anti-D made by individuals of different phenotypic categories
- Transfusion history:
- Pregnancy history:
- Anti-D Ig history:
- Context:
- Hemolytic consequences:
- Comment:
TIPPETT P et al. Vox Sang (1962)
Tippett P et al. J Immunogenet (1990) (see
Antibodies in D negative recipients
Alloimmunization in recipients: expected to be possible, see phenotype data
Reports
Summary: NA, NA (last update: Aug. 25, 2020)Detailed reports
-
4/342 out of 342 D samples sent for D phenotyping (76 were too weak for epitope determination and classification, only half of the remaining could be classified)
(probably overlaps with
13921349 ) -
10 to 30 samples among the propositi tested by the authors Black
(probably overlaps with
13921349 ) -
1 sample among D positive samples with anti-D Puerto Rican
(probably also included in
8740002 ) -
2 samples among D negative samples with anti-D Black
(probably also included in
8740002 )
Allele or phenotype frequency
Structure mapping
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References
- TIPPETT P et al. Observations on subdivisions of the Rh antigen D. Vox Sang, 1962. [Citation] [RHeference]
- CHOWN B et al. A "D-POSITIVE" MAN WHO PRODUCED ANTI-D. Vox Sang, 1963. [Citation] [RHeference]
- Tippett P et al. Monoclonal antibodies against Rh and Rh related antigens. J Immunogenet, 1990. [Citation] [RHeference]
- Lomas C et al. Further complexities of the Rh antigen D disclosed by testing category DII cells with monoclonal anti-D. Transfus Med, 1993. [Citation] [RHeference]
- Gorick B et al. Quantitation of D sites on selected 'weak D' and 'partial D' red cells. Vox Sang, 1993. [Citation] [RHeference]
- Jones JW et al. Quantitation of Rh D antigen sites on weak D and D variant red cells by flow cytometry. Vox Sang, 1996. [Citation] [RHeference]
- Cartron JP et al. Tentative model for the mapping of D epitopes on the RhD polypeptide. Transfus Clin Biol, 1996. [Citation] [RHeference]
- Tippett P et al. The Rh antigen D: partial D antigens and associated low incidence antigens. Vox Sang, 1996. [Citation] [RHeference]
- Jones J et al. Selection of monoclonal antibodies for the identification of D variants: ability to detect weak D and to split epD2, epD5 and epD6/7. Vox Sang, 1996. [Citation] [RHeference]
- Avent ND et al. Evidence of genetic diversity underlying Rh D-, weak D (Du), and partial D phenotypes as determined by multiplex polymerase chain reaction analysis of the RHD gene. Blood, 1997. [Citation] [RHeference]
- Reid ME et al. Use of LOR-15C9 monoclonal anti-D to differentiate erythrocytes with the partial DvI antigen from those with either partial D antigens or weak D antigens. Immunohematology, 1998. [Citation] [RHeference]
- Maaskant-van Wijk PA et al. Genotyping of RHD by multiplex polymerase chain reaction analysis of six RHD-specific exons. Transfusion, 1998. [Citation] [RHeference]
- Reid ME et al. DAK, a new low-incidence antigen in the Rh blood group system. Transfusion, 2003. [Citation] [RHeference]
- Kulkarni S et al. Frequency of partial D in Western India. Transfus Med, 2008. [Citation] [RHeference]
- Haer-Wigman L et al. RHD and RHCE variant and zygosity genotyping via multiplex ligation-dependent probe amplification. Transfusion, 2013. [Citation] [RHeference]
- von Zabern I et al. D category IV: a group of clinically relevant and phylogenetically diverse partial D. Transfusion, 2013. [Citation] [RHeference]
- Floch A et al. Comment from Rheference Online ressource, 2020. — Online ressource — [RHeference]
Last update: June 16, 2020