DVII - phenotypic description
(ISBT table: not listed)
This entry is a phenotypic characterization.
DVII - phenotypic description,
Molecular data
Nucleotides:
Amino acids:
Hybrid allele encompassing at least one RHCE exon:
NA
Comments on the molecular basis:
- phenotype associated with RHef00107
- molecular typing not specified, assumed phenotypic characterization
Extracellular position of one or more amino acid substitutions:
Splicing:
Unconventional prediction methods:
Phenotype
Main D phenotype: variable/discrepant (last update: June 16, 2020)Reports by D phenotype
Other RH phenotypes: RH:-3, -4, -8, -9, -10, -11, -20, -23, -30, -32, -36, 40, -50, -54,
Serology with monoclonal anti-D
- with cross-testing table between different D categories
- 1 anti-D non-reactive with variant, out of 10 anti-D tested (9 monoclonal IgG)
- 5 samples tested; reactive (2+ to 4+) with LOR-15C9 Ab
- 0 monoclonal IgG anti-D non-reactive with variant, out of 5 monoclonal anti-D tested; one polyclonal anti-D was also tested (Table 1)
- epitope pattern (Table 1)
- tested with monoclonal anti-D; epitope pattern
- reactivity with monoclonal anti-D, tested by different laboratories (Table 1)
- reactivity pattern with 6 monoclonal anti-D
Antigen Density (Ag/RBC)
- 7500 Ag/RBC, 1 sample, tested with 1 monoclonal anti-D
- 4200 Ag/RBC, 1 sample, tested with 1 monoclonal anti-D
- 2400 Ag/RBC, 1 sample, tested with 1 monoclonal anti-D
- range: 1400 - 5300 Ag/RBC, 6 R1r samples, tested with 5 monoclonal anti-D (H27, BRAD5, BRAD7, 2B6, BRAD3), and one polyclonal anti-D (Table 1, Figure 3)
More phenotype data
Rhesus Similarity Index
Haplotype
Main CcEe phenotype association: Ce (last update: June 16, 2020)Alloimmunization
Antibodies in carriers
Antibody specificity: D (RH1)
Summary: not relevant, see types or alleles (last update: Aug. 25, 2020)Detailed information
-
Tippett P et al. Vox Sang (1996)
- Ab specificity: D (RH1)
- Number (auto- or allo-): 8
- Number listed as allo-:
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT:
- Autologuous control:
- Elution:
- Autoadsorption:
- Titer:
- Was anti-LW excluded?:
- Other antibodies detected: strong anti-E in the 4 individuals with a transfusion history
- Cross matches (with Ab and RBCs from different partial types):
- Transfusion history:
- Pregnancy history:
- Anti-D Ig history:
- Context:
- Hemolytic consequences:
- Comment: 2 were male patients who had never been transfused and in 4 others a weak anti-D is accompanied by a strong anti-E
-
Ansart-Pirenne H et al. Transfusion (2004)
- Ab specificity: D (RH1)
- Number (auto- or allo-): 3
- Number listed as allo-:
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT:
- Autologuous control:
- Elution:
- Autoadsorption:
- Titer:
- Was anti-LW excluded?:
- Other antibodies detected:
- Cross matches (with Ab and RBCs from different partial types):
- Transfusion history:
- Pregnancy history:
- Anti-D Ig history:
- Context:
- Hemolytic consequences:
- Comment:
Antibodies in D negative recipients
Alloimmunization in recipients: expected to be possible, see phenotype data
Reports
Summary: NA, NA (last update: Aug. 25, 2020)Detailed reports
- 15/342 out of 342 D samples sent for D phenotyping (76 were too weak for epitope determination and classification, only half of the remaining could be classified)
- >30 samples among the propositi tested by the authors White
- 8/168 among 168 samples referred for weak D phenotype and/or allo anti-D in D positive individuals, 137 were characterized in the study (70 by serology, 67 by molecular analysis, 31 could not be typed because serologic typing was inconclusive and molecular typing could not be performed) in the French population (Caucasian)
- 2/55162 among samples C or E positive and positive for "Du test" (55162 samples were typed; 314 were ddCcee, with 63 positive for "Du test" and 154 were ddccEe, with 30 positive for "Du test"; of the 93 "Du positive", 60 underwent molecular analysis) in the French population
- 1/10000 screening of D positive individuals Indian (West India)
- 80/78156 donations, of which 60965 had D positive phenotype. 80 donations, from 71 donors, had RHef00582, detected by serologic screening in the German population, southwestern Germany
Allele or phenotype frequency
Structure mapping
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References
- Lewis M et al. Assignment of the red cell antigen, Targett (Rh40), to the Rh blood group system. Am J Hum Genet, 1979. [Citation] [RHeference]
- Lomas C et al. Demonstration of seven epitopes on the Rh antigen D using human monoclonal anti-D antibodies and red cells from D categories. Vox Sang, 1989. [Citation] [RHeference]
- Tippett P et al. Monoclonal antibodies against Rh and Rh related antigens. J Immunogenet, 1990. [Citation] [RHeference]
- Gorick B et al. Quantitation of D sites on selected 'weak D' and 'partial D' red cells. Vox Sang, 1993. [Citation] [RHeference]
- Jones JW et al. Quantitation of Rh D antigen sites on weak D and D variant red cells by flow cytometry. Vox Sang, 1996. [Citation] [RHeference]
- Cartron JP et al. Tentative model for the mapping of D epitopes on the RhD polypeptide. Transfus Clin Biol, 1996. [Citation] [RHeference]
- Tippett P et al. The Rh antigen D: partial D antigens and associated low incidence antigens. Vox Sang, 1996. [Citation] [RHeference]
- Jones J et al. Selection of monoclonal antibodies for the identification of D variants: ability to detect weak D and to split epD2, epD5 and epD6/7. Vox Sang, 1996. [Citation] [RHeference]
- Avent ND et al. Evidence of genetic diversity underlying Rh D-, weak D (Du), and partial D phenotypes as determined by multiplex polymerase chain reaction analysis of the RHD gene. Blood, 1997. [Citation] [RHeference]
- Reid ME et al. Use of LOR-15C9 monoclonal anti-D to differentiate erythrocytes with the partial DvI antigen from those with either partial D antigens or weak D antigens. Immunohematology, 1998. [Citation] [RHeference]
- Reid ME et al. DAK, a new low-incidence antigen in the Rh blood group system. Transfusion, 2003. [Citation] [RHeference]
- Ansart-Pirenne H et al. RhD variants in Caucasians: consequences for checking clinically relevant alleles. Transfusion, 2004. [Citation] [RHeference]
- Noizat-Pirenne F et al. Weak D phenotypes and transfusion safety: where do we stand in daily practice? Transfusion, 2007. [Citation] [RHeference]
- Kulkarni S et al. Frequency of partial D in Western India. Transfus Med, 2008. [Citation] [RHeference]
- von Zabern I et al. D category IV: a group of clinically relevant and phylogenetically diverse partial D. Transfusion, 2013. [Citation] [RHeference]
- Haer-Wigman L et al. RHD and RHCE variant and zygosity genotyping via multiplex ligation-dependent probe amplification. Transfusion, 2013. [Citation] [RHeference]
- Floch A et al. Comment from Rheference Online ressource, 2020. — Online ressource — [RHeference]
Last update: June 16, 2020