DVI - phenotypic description
(ISBT table: not listed)
This entry is a phenotypic characterization.
DVI - phenotypic description,
Molecular data
Nucleotides:
Amino acids:
Hybrid allele encompassing at least one RHCE exon:
NA
Comments on the molecular basis:
- molecular characterization of DVI phenotypes
- phenotype associated with RHef00102 and RHef00103
- molecular typing not specified, assumed phenotypic characterization
Extracellular position of one or more amino acid substitutions:
Splicing:
Unconventional prediction methods:
Phenotype
Main D phenotype: variable/discrepant (last update: June 16, 2020)Reports by D phenotype
Other RH phenotypes: RH:-2, -3, -4, -23, -30, -40, -50, 52, -54, -52,
Serology with monoclonal anti-D
- with cross-testing table between different D categories
- 6 anti-D non-reactive with variant, out of 10 anti-D tested (9 monoclonal IgG)
- 21 samples tested; reactive (2+ to 4+) with LOR-15C9 Ab, considerable variation of reaction strengths; stronger reactions with LOR-15C9 than with HAM-A, RUM-1 and MAD-2 Ab
- 3 (and 1 variable) anti-D non-reactive with variant, out of 5 monoclonal anti-D tested; one polyclonal anti-D was also tested (Table 1)
- epitope pattern (Table 1)
- tested with monoclonal anti-D; epitope pattern
- 9 samples tested; positive reaction with about 35% anti-D tested (Ab not detailed) (Table 1)
- reactivity with monoclonal anti-D, tested by different laboratories (Table 1)
- samples of different RHCE phenotypes tested with 3 monoclonal anti-D (Table 5)
Antigen Density (Ag/RBC)
- range: 1700 - 3200 Ag/RBC, with a polyclonal anti-D
- <500 Ag/RBC, 4 samples, tested with 1 monoclonal anti-D
- 400 Ag/RBC, 1 R0r sample, tested with 5 monoclonal anti-D (H27, BRAD5, BRAD7, 2B6, BRAD3), and one polyclonal anti-D (Table 1, Figure 3)
- range: 1600 - 2900 Ag/RBC, 10 R1r samples, tested with 5 monoclonal anti-D (H27, BRAD5, BRAD7, 2B6, BRAD3), and one polyclonal anti-D (Table 1, Figure 3)
- 300 Ag/RBC, 4 R2r samples, tested with 5 monoclonal anti-D (H27, BRAD5, BRAD7, 2B6, BRAD3), and one polyclonal anti-D (Table 1, Figure 3)
More phenotype data
Rhesus Similarity Index
Haplotype
Main CcEe phenotype association: Ce (last update: June 16, 2020)ce | Ce | cE | CE | |
---|---|---|---|---|
ce | 1 | 35 | 9 | 0 |
Ce | 3 | 0 | 0 | |
cE | 0 | 0 | ||
CE | 0 |
Reports by CcEe phenotype
Reports by allele association
Alloimmunization
Antibodies in carriers
Antibody specificity: D (RH1)
Summary: not relevant, see types or alleles (last update: Aug. 25, 2020)Detailed information
-
Ansart-Pirenne H et al. Transfusion (2004)
- Ab specificity: D (RH1)
- Number (auto- or allo-): 2
- Number listed as allo-:
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT:
- Autologuous control:
- Elution:
- Autoadsorption:
- Titer:
- Was anti-LW excluded?:
- Other antibodies detected:
- Cross matches (with Ab and RBCs from different partial types):
- Transfusion history:
- Pregnancy history:
- Anti-D Ig history:
- Context:
- Hemolytic consequences:
- Comment:
-
Lomas C et al. Transfus Med (1993)
(Table 2)
- Ab specificity: D (RH1)
- Number (auto- or allo-):
- Number listed as allo-: 17 (?)
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT:
- Autologuous control:
- Elution:
- Autoadsorption:
- Titer:
- Was anti-LW excluded?:
- Other antibodies detected:
- Cross matches (with Ab and RBCs from different partial types): cross-matching of RBCs and anti-D made by individuals of different phenotypic categories
- Transfusion history:
- Pregnancy history:
- Anti-D Ig history:
- Context:
- Hemolytic consequences:
- Comment:
TIPPETT P et al. Vox Sang (1962)
Tippett P et al. J Immunogenet (1990) (see
-
Lacey PA et al. Transfusion (1983)
- Ab specificity: D (RH1)
- Number (auto- or allo-):
- Number listed as allo-: 1
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT: negative
- Autologuous control: negative
- Elution:
- Autoadsorption:
- Titer: 4096
- Was anti-LW excluded?:
- Other antibodies detected:
- Cross matches (with Ab and RBCs from different partial types): "anti-D sera from seven Category DVI or Du variants were nonreactive with her red cells"
- Transfusion history: no transfusion history
- Pregnancy history:
- Anti-D Ig history:
- Context:
- Hemolytic consequences: severe HDFN with neonatal death
- Comment:
-
Cannon M et al. Obstet Gynecol (2003)
- Ab specificity: D (RH1)
- Number (auto- or allo-):
- Number listed as allo-: 1
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT:
- Autologuous control:
- Elution:
- Autoadsorption:
- Titer: 64
- Was anti-LW excluded?:
- Other antibodies detected:
- Cross matches (with Ab and RBCs from different partial types):
- Transfusion history: no transfusion history
- Pregnancy history:
- Anti-D Ig history:
- Context:
- Hemolytic consequences: severe HDFN with death at J6
- Comment:
Antibodies in D negative recipients
Alloimmunization in recipients: expected to be possible, see phenotype data
Reports
Summary: NA, NA (last update: Aug. 25, 2020)Detailed reports
- 2/5382541 donor samples tested, 32 were considered to have "partial D phenotype" Japanese
-
46/342 out of 342 D samples sent for D phenotyping (76 were too weak for epitope determination and classification, only half of the remaining could be classified)
(probably overlaps with
13921349 ) -
>30 samples among the propositi tested by the authors White ##### Japanese
(probably overlaps with
13921349 ) - 6/42987 donors German (South-West: Baden-Wurttemberg)
- 6/24632 (in this study 92 donors had weak D phenotype) D positive donors tested with anti-D BS226 German (South-West: Baden-Wurttemberg)
- 37/168 among 168 samples referred for weak D phenotype and/or allo anti-D in D positive individuals, 137 were characterized in the study (70 by serology, 67 by molecular analysis, 31 could not be typed because serologic typing was inconclusive and molecular typing could not be performed) in the French population (Caucasian)
- 1 sample non invasive fetal RHD detection study in the Argentinean population (Rosario)
-
9 samples among D positive samples with anti-D Caucasian
(probably also included in
8740002 )
Allele or phenotype frequency
- 1/15 (CI: 1/7 - 1/35) estimated frequency of DVI phenotype in donors with weak D phenotype German (South-West: Baden-Wurttemberg)
- 1/4105 (CI: 1/1922 - 1/9426) estimated frequency of DVI phenotype in D positive (weak or not) donors German (South-West: Baden-Wurttemberg)
- 1/6214 (CI: 1/3667 - 1/11153) estimated frequency of DVI phenotype in donors (both D positive and D negative) German (South-West: Baden-Wurttemberg)
Structure mapping
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References
- TIPPETT P et al. Observations on subdivisions of the Rh antigen D. Vox Sang, 1962. [Citation] [RHeference]
- Lacey PA et al. Fatal hemolytic disease of a newborn due to anti-D in an Rh-positive Du variant mother. Transfusion, 1983. [Citation] [RHeference]
- Merry AH et al. Variation in the level of Rh(D) antigen expression. Transfusion, 1988. [Citation] [RHeference]
- Lomas C et al. Demonstration of seven epitopes on the Rh antigen D using human monoclonal anti-D antibodies and red cells from D categories. Vox Sang, 1989. [Citation] [RHeference]
- Tippett P et al. Monoclonal antibodies against Rh and Rh related antigens. J Immunogenet, 1990. [Citation] [RHeference]
- Leader KA et al. Human monoclonal anti-D with reactivity against category DVI cells used in blood grouping and determination of the incidence of the category DVI phenotype in the DU population. Vox Sang, 1990. [Citation] [RHeference]
- Okubo Y et al. Partial D antigens disclosed by a monoclonal anti-D in Japanese blood donors. Transfusion, 1991. [Citation] [RHeference]
- Lomas C et al. Further complexities of the Rh antigen D disclosed by testing category DII cells with monoclonal anti-D. Transfus Med, 1993. [Citation] [RHeference]
- Gorick B et al. Quantitation of D sites on selected 'weak D' and 'partial D' red cells. Vox Sang, 1993. [Citation] [RHeference]
- Wagner F et al. [Occurrence of D category VI in blood donors in Baden-Württemberg]. Beitr Infusionsther Transfusionsmed, 1994. [Citation] [RHeference]
- Wagner FF et al. Frequencies of the blood groups ABO, Rhesus, D category VI, Kell, and of clinically relevant high-frequency antigens in south-western Germany. Infusionsther Transfusionsmed, 1995. [Citation] [RHeference]
- Jones JW et al. Quantitation of Rh D antigen sites on weak D and D variant red cells by flow cytometry. Vox Sang, 1996. [Citation] [RHeference]
- Cartron JP et al. Tentative model for the mapping of D epitopes on the RhD polypeptide. Transfus Clin Biol, 1996. [Citation] [RHeference]
- Jones J et al. Selection of monoclonal antibodies for the identification of D variants: ability to detect weak D and to split epD2, epD5 and epD6/7. Vox Sang, 1996. [Citation] [RHeference]
- Tippett P et al. The Rh antigen D: partial D antigens and associated low incidence antigens. Vox Sang, 1996. [Citation] [RHeference]
- Avent ND et al. Evidence of genetic diversity underlying Rh D-, weak D (Du), and partial D phenotypes as determined by multiplex polymerase chain reaction analysis of the RHD gene. Blood, 1997. [Citation] [RHeference]
- Reid ME et al. Use of LOR-15C9 monoclonal anti-D to differentiate erythrocytes with the partial DvI antigen from those with either partial D antigens or weak D antigens. Immunohematology, 1998. [Citation] [RHeference]
- Wagner FF et al. Three molecular structures cause rhesus D category VI phenotypes with distinct immunohematologic features. Blood, 1998. [Citation] [RHeference]
- Wagner FF et al. A D(V)-like phenotype is obliterated by A226P in the partial D DBS. Transfusion, 2001. [Citation] [RHeference]
- Cannon M et al. Fatal hydrops fetalis caused by anti-D in a mother with partial D. Obstet Gynecol, 2003. [Citation] [RHeference]
- Reid ME et al. DAK, a new low-incidence antigen in the Rh blood group system. Transfusion, 2003. [Citation] [RHeference]
- Ansart-Pirenne H et al. RhD variants in Caucasians: consequences for checking clinically relevant alleles. Transfusion, 2004. [Citation] [RHeference]
- Ye L et al. Partial D phenotypes and genotypes in the Chinese population. Transfusion, 2012. [Citation] [RHeference]
- Haer-Wigman L et al. RHD and RHCE variant and zygosity genotyping via multiplex ligation-dependent probe amplification. Transfusion, 2013. [Citation] [RHeference]
- Boggione CT et al. Genotyping approach for non-invasive foetal RHD detection in an admixed population. Blood Transfus, 2017. [Citation] [RHeference]
- Floch A et al. Comment from Rheference Online ressource, 2020. — Online ressource — [RHeference]
Last update: June 16, 2020