DII - phenotypic description
(ISBT table: not listed)
This entry is a phenotypic characterization.
DII - phenotypic description,
Molecular data
Nucleotides:
Amino acids:
Hybrid allele encompassing at least one RHCE exon:
NA
Comments on the molecular basis:
- see also "Additional comments" section on the RhesusBase http://www.rhesusbase.info/RHDDII.htm
- link between the DII phenotype and RHef00055
Extracellular position of one or more amino acid substitutions:
Splicing:
Unconventional prediction methods:
Phenotype
Main D phenotype: variable/discrepant (last update: June 16, 2020)Reports by D phenotype
- Discrepant D phenotype (negative or positive depending on anti-D reagents and techniques)
- Molecular DNU and DII have the same serologic "DII" profile
Other RH phenotypes: RH:-3, -23, -30, -40, -50,
Serology with monoclonal anti-D
- cross-testing table between different D categories
- 1 sample tested; weakly reactive (1+ to 2+) with LOR-15C9 Ab, strongly agglutinated by HAM-A, RUM-1 and MAD-2 Ab
- 0 monoclonal IgG anti-D non-reactive with variant, out of 5 monoclonal anti-D tested; one polyclonal anti-D was also tested (Table 1)
- epitope pattern (Table 1)
- tested with monoclonal anti-D; epitope pattern
- epitope pattern and cross-testing
- 1 sample tested; positive reaction with all of 85 anti-D tested (Ab not detailed) (Table 1)
- reactivity pattern with 6 monoclonal anti-D
Antigen Density (Ag/RBC)
More phenotype data
Rhesus Similarity Index
Haplotype
Main CcEe phenotype association: Ce (last update: Feb. 18, 2020)Alloimmunization
Antibodies in carriers
Antibody specificity: D (RH1)
Summary: not relevant, see types or alleles (last update: Aug. 25, 2020)Detailed information
-
TIPPETT P et al. Vox Sang (1962)
- Ab specificity: D (RH1)
- Number (auto- or allo-):
- Number listed as allo-: 1
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT:
- Autologuous control:
- Elution:
- Autoadsorption:
- Titer:
- Was anti-LW excluded?:
- Other antibodies detected:
- Cross matches (with Ab and RBCs from different partial types): cross-matching of RBCs and anti-D made by individuals of different phenotypic categories
- Transfusion history:
- Pregnancy history:
- Anti-D Ig history:
- Context:
- Hemolytic consequences:
- Comment:
Tippett P et al. J Immunogenet (1990) (see
Lomas C et al. Transfus Med (1993) (Table 2)
Antibodies in D negative recipients
Alloimmunization in recipients: expected to be possible, see phenotype data
Reports
Summary: NA, NA (last update: Aug. 25, 2020)Structure mapping
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References
- TIPPETT P et al. Observations on subdivisions of the Rh antigen D. Vox Sang, 1962. [Citation] [RHeference]
- Tippett P et al. Monoclonal antibodies against Rh and Rh related antigens. J Immunogenet, 1990. [Citation] [RHeference]
- Lomas C et al. Further complexities of the Rh antigen D disclosed by testing category DII cells with monoclonal anti-D. Transfus Med, 1993. [Citation] [RHeference]
- Cartron JP et al. Tentative model for the mapping of D epitopes on the RhD polypeptide. Transfus Clin Biol, 1996. [Citation] [RHeference]
- Jones JW et al. Quantitation of Rh D antigen sites on weak D and D variant red cells by flow cytometry. Vox Sang, 1996. [Citation] [RHeference]
- Tippett P et al. The Rh antigen D: partial D antigens and associated low incidence antigens. Vox Sang, 1996. [Citation] [RHeference]
- Jones J et al. Selection of monoclonal antibodies for the identification of D variants: ability to detect weak D and to split epD2, epD5 and epD6/7. Vox Sang, 1996. [Citation] [RHeference]
- Avent ND et al. Molecular basis of the D variant phenotypes DNU and DII allows localization of critical amino acids required for expression of Rh D epitopes epD3, 4 and 9 to the sixth external domain of the Rh D protein. Br J Haematol, 1997. [Citation] [RHeference]
- Avent ND et al. Evidence of genetic diversity underlying Rh D-, weak D (Du), and partial D phenotypes as determined by multiplex polymerase chain reaction analysis of the RHD gene. Blood, 1997. [Citation] [RHeference]
- Reid ME et al. Use of LOR-15C9 monoclonal anti-D to differentiate erythrocytes with the partial DvI antigen from those with either partial D antigens or weak D antigens. Immunohematology, 1998. [Citation] [RHeference]
- Kulkarni S et al. Frequency of partial D in Western India. Transfus Med, 2008. [Citation] [RHeference]
- Wagner FF and Flegel WA et al. The Human RhesusBase Online ressource, 2011. — Online ressource — [RHeference]
- Floch A et al. Comment from Rheference Online ressource, 2020. — Online ressource — [RHeference]
Last update: June 16, 2020