DVa - phenotypic description
(ISBT table: not listed)
This entry is a phenotypic characterization.
DVa - phenotypic description,
Molecular data
Nucleotides:
Amino acids:
Hybrid allele encompassing at least one RHCE exon:
NA
Comments on the molecular basis:
- molecular typing of several phenotypic DVa and Dva-like samples
- PCR pattern, exons 3, 4, 5, 6, 7 and 9 and epitope pattern
- molecular typing of several phenotypic DVa and Dva-like samples; residue 233 seems key to the DVa phenotype
- molecular typing not specified, assumed phenotypic characterization
Extracellular position of one or more amino acid substitutions:
Splicing:
Unconventional prediction methods:
Phenotype
Main D phenotype: variable/discrepant (last update: May 6, 2020)Reports by D phenotype
Other RH phenotypes: RH:-3, 23, -30, -32, -40, -50, -52, -54, 56,
Serology with monoclonal anti-D
- with cross-testing table between different D categories
- 2 monoclonal IgG anti-D non-reactive with variant, out of 13 anti-D tested (used as control, Data S1)
- 2 anti-D non-reactive with variant, out of 10 anti-D tested (9 monoclonal IgG)
- 7 samples tested; reactive (2+ to 4+) with LOR-15C9 Ab, considerable variation of reaction strengths
- reaction with 1 anti-D variable, out of 5 monoclonal anti-D tested; one polyclonal anti-D was also tested (Table 1)
- epitope pattern
(data reproduced from
9018785 ) (Table 1) - tested with monoclonal anti-D; epitope pattern
- 1 sample tested; positive reaction with 44 of 52 anti-D tested (Ab not detailed) (Table 1)
- reactivity with monoclonal anti-D, tested by different laboratories (Table 1)
- DVa category in the father and child was confirmed by cross-testing with anti-D made by DVa individuals (Table 2)
Antigen Density (Ag/RBC)
- <500 Ag/RBC, 4 samples, tested with 1 monoclonal anti-D
- range: 600 - 6000 Ag/RBC, 3 R0r samples, tested with 5 monoclonal anti-D (H27, BRAD5, BRAD7, 2B6, BRAD3), and one polyclonal anti-D (Table 1, Figure 3)
- range: 4300 - 12500 Ag/RBC, 2 R1r samples, tested with 5 monoclonal anti-D (H27, BRAD5, BRAD7, 2B6, BRAD3), and one polyclonal anti-D (Table 1, Figure 3)
More phenotype data
Rhesus Similarity Index
Haplotype
Main CcEe phenotype association: Ce and ce (last update: May 3, 2020)Alloimmunization
Antibodies in carriers
Antibody specificity: D (RH1)
Summary: not relevant, see types or alleles (last update: Aug. 25, 2020)Detailed information
-
Ansart-Pirenne H et al. Transfusion (2004)
- Ab specificity: D (RH1)
- Number (auto- or allo-): 1
- Number listed as allo-:
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT:
- Autologuous control:
- Elution:
- Autoadsorption:
- Titer:
- Was anti-LW excluded?:
- Other antibodies detected:
- Cross matches (with Ab and RBCs from different partial types):
- Transfusion history:
- Pregnancy history:
- Anti-D Ig history:
- Context:
- Hemolytic consequences:
- Comment:
-
Lomas C et al. Transfus Med (1993)
(Table 2)
- Ab specificity: D (RH1)
- Number (auto- or allo-):
- Number listed as allo-: 2
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT:
- Autologuous control:
- Elution:
- Autoadsorption:
- Titer:
- Was anti-LW excluded?:
- Other antibodies detected:
- Cross matches (with Ab and RBCs from different partial types): cross-matching of RBCs and anti-D made by individuals of different phenotypic categories
- Transfusion history:
- Pregnancy history:
- Anti-D Ig history:
- Context:
- Hemolytic consequences:
- Comment:
TIPPETT P et al. Vox Sang (1962)
Tippett P et al. J Immunogenet (1990) (see
Antibodies in D negative recipients
Alloimmunization in recipients: yes, see detailed reports and phenotype data
-
Mayne K et al. Br J Haematol (1990)
- Ab specificity: D (RH1)
- Number of cases: 1
- Exposures: pregnancy
- Imputability:
- Comment: anti-D developped during second pregnancy of a D negative woman who had received anti-D immunoprophylaxy after the birth of her first child; no history of miscarriages, blood transfusions or autoimmune disorders; Ab screening test negative at 13 weeks gestation, positive at 28; the neonate had weakly positive DAT, became jaundiced and required phototherapy but no transfusion
Reports
Summary: NA, NA (last update: Aug. 25, 2020)Detailed reports
- 1/5382541 donor samples tested, 32 were considered to have "partial D phenotype" Japanese
- 2 samples (father and child) pregnancy follow-up of a D negative mother who developped anti-D Caucasian, reported by a UK lab
-
30/342 out of 342 D samples sent for D phenotyping (76 were too weak for epitope determination and classification, only half of the remaining could be classified)
(probably overlaps with
13921349 ) -
>30 samples among the propositi tested by the authors White ##### Black ##### Japanese
(probably overlaps with
13921349 ) -
1 sample among D positive samples with anti-D Caucasian
(probably also included in
8740002 ) - 2/168 among 168 samples referred for weak D phenotype and/or allo anti-D in D positive individuals, 137 were characterized in the study (70 by serology, 67 by molecular analysis, 31 could not be typed because serologic typing was inconclusive and molecular typing could not be performed) in the French population (Caucasian)
Allele or phenotype frequency
Structure mapping
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References
- TIPPETT P et al. Observations on subdivisions of the Rh antigen D. Vox Sang, 1962. [Citation] [RHeference]
- Lomas C et al. Demonstration of seven epitopes on the Rh antigen D using human monoclonal anti-D antibodies and red cells from D categories. Vox Sang, 1989. [Citation] [RHeference]
- Tippett P et al. Monoclonal antibodies against Rh and Rh related antigens. J Immunogenet, 1990. [Citation] [RHeference]
- Mayne K et al. Rh immunization by the partial D antigen of category DVa. Br J Haematol, 1990. [Citation] [RHeference]
- Okubo Y et al. Partial D antigens disclosed by a monoclonal anti-D in Japanese blood donors. Transfusion, 1991. [Citation] [RHeference]
- Lomas C et al. Abolition of the DVc subcategory. Transfusion, 1993. [Citation] [RHeference]
- Lomas C et al. Further complexities of the Rh antigen D disclosed by testing category DII cells with monoclonal anti-D. Transfus Med, 1993. [Citation] [RHeference]
- Gorick B et al. Quantitation of D sites on selected 'weak D' and 'partial D' red cells. Vox Sang, 1993. [Citation] [RHeference]
- Jones J et al. Selection of monoclonal antibodies for the identification of D variants: ability to detect weak D and to split epD2, epD5 and epD6/7. Vox Sang, 1996. [Citation] [RHeference]
- Scott M et al. Rh serology--coordinator's report. Transfus Clin Biol, 1996. [Citation] [RHeference]
- Jones JW et al. Quantitation of Rh D antigen sites on weak D and D variant red cells by flow cytometry. Vox Sang, 1996. [Citation] [RHeference]
- Cartron JP et al. Tentative model for the mapping of D epitopes on the RhD polypeptide. Transfus Clin Biol, 1996. [Citation] [RHeference]
- Tippett P et al. The Rh antigen D: partial D antigens and associated low incidence antigens. Vox Sang, 1996. [Citation] [RHeference]
- Avent ND et al. Evidence of genetic diversity underlying Rh D-, weak D (Du), and partial D phenotypes as determined by multiplex polymerase chain reaction analysis of the RHD gene. Blood, 1997. [Citation] [RHeference]
- Maaskant-van Wijk PA et al. Genotyping of RHD by multiplex polymerase chain reaction analysis of six RHD-specific exons. Transfusion, 1998. [Citation] [RHeference]
- Reid ME et al. Use of LOR-15C9 monoclonal anti-D to differentiate erythrocytes with the partial DvI antigen from those with either partial D antigens or weak D antigens. Immunohematology, 1998. [Citation] [RHeference]
- Reid ME et al. Two examples of an inseparable antibody that reacts equally well with DW+ and Rh32+ red blood cells. Vox Sang, 1998. [Citation] [RHeference]
- Omi T et al. The genomic organization of the partial D category DVa: the presence of a new partial D associated with the DVa phenotype. Biochem Biophys Res Commun, 1999. [Citation] [RHeference]
- Hyodo H et al. Polymorphisms of RhD(Va) and a new RhD(Va)-like variant found in Japanese individuals. Vox Sang, 2000. [Citation] [RHeference]
- Legler TJ et al. D(Va) category phenotype and genotype in Japanese families. Vox Sang, 2000. [Citation] [RHeference]
- Müller TH et al. PCR screening for common weak D types shows different distributions in three Central European populations. Transfusion, 2001. [Citation] [RHeference]
- Wagner FF et al. A D(V)-like phenotype is obliterated by A226P in the partial D DBS. Transfusion, 2001. [Citation] [RHeference]
- Reid ME et al. DAK, a new low-incidence antigen in the Rh blood group system. Transfusion, 2003. [Citation] [RHeference]
- Westhoff CM et al. A new hybrid RHCE gene (CeNR) is responsible for expression of a novel antigen. Transfusion, 2004. [Citation] [RHeference]
- Ansart-Pirenne H et al. RhD variants in Caucasians: consequences for checking clinically relevant alleles. Transfusion, 2004. [Citation] [RHeference]
- Takeuchi-Baba C et al. Production of RBC autoantibody mimicking anti-D specificity following transfusion in a patient with weak D Type 15. Transfusion, 2019. [Citation] [RHeference]
- Floch A et al. Comment from Rheference Online ressource, 2020. — Online ressource — [RHeference]
Last update: June 16, 2020