DBT - phenotypic description
(ISBT table: not listed)
This entry is a phenotypic characterization.
DBT - phenotypic description,
Phenotype
Main D phenotype: variable/discrepant (last update: June 16, 2020)Reports by D phenotype
Other RH phenotypes: RH:-2, -3, -4, -10, 12, -20, -23, -30, 32, -33, -36, -37, -40, -48, -50, -54, -12,
- RH:-2 inferred from the reported RHCE phenotypes of the carriers
- RH:-3 inferred from the reported RHCE phenotypes of the carriers
- RH:-4 inferred from the reported RHCE phenotypes of the carriers
- RH:-10
- RH:12 with most samples, but one "antigen present as judged by some antibodies but absent as judged by others"
- RH:-20
- RH:-23
- RH:-30
- RH:32 general association, not tested in this study weaker expression than RN cells
- RH:-33
- RH:-36
- RH:-37
- RH:-40
- RH:-48
- RH:-50
- RH:-54
- RH:-12 one sample "antigen present as judged by some antibodies but absent as judged by others"
Serology with monoclonal anti-D
- with cross-testing table between different D categories
- 42 anti-D non-reactive with variant, out of 49 monoclonal anti-D tested
- 7 anti-D non-reactive with variant, out of 10 anti-D tested (9 monoclonal IgG)
- 2 samples tested; non-reactive with LOR-15C9 Ab
- 4 anti-D non-reactive with variant, out of 5 monoclonal anti-D tested; one polyclonal anti-D was also tested (Table 1)
- epitope pattern (Table 1)
- testing with monoclonal anti-D; epitope pattern
- reactivity pattern with 6 monoclonal anti-D
Antigen Density (Ag/RBC)
More phenotype data
Rhesus Similarity Index
Haplotype
Main CcEe phenotype association: Ce (last update: Feb. 18, 2020)ce | Ce | cE | CE | |
---|---|---|---|---|
ce | 1 | 3 | 0 | 0 |
Ce | 8 | 0 | 0 | |
cE | 0 | 0 | ||
CE | 0 |
Reports by CcEe phenotype
Reports by allele association
Alloimmunization
Antibodies in carriers
Antibody specificity: D (RH1)
Summary: not relevant, see types or alleles (last update: Aug. 25, 2020)Detailed information
-
Wallace M et al. Transfus Med (1997)
- Ab specificity: D (RH1)
- Number (auto- or allo-): 3
- Number listed as allo-:
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT:
- Autologuous control:
- Elution:
- Autoadsorption:
- Titer:
- Was anti-LW excluded?:
- Other antibodies detected:
- Cross matches (with Ab and RBCs from different partial types):
- Transfusion history:
- Pregnancy history:
- Anti-D Ig history:
- Context:
- Hemolytic consequences:
- Comment: 3 cases in two families, proband 8 from 8652401 is not counted twice
-
M. J. Rodrigues et al. Transfusion Medicine (2002)
- Ab specificity: D (RH1)
- Number (auto- or allo-): 1
- Number listed as allo-:
- Number listed as auto-:
- Number of carriers of the allele assessed:
- DAT:
- Autologuous control:
- Elution:
- Autoadsorption:
- Titer:
- Was anti-LW excluded?:
- Other antibodies detected:
- Cross matches (with Ab and RBCs from different partial types):
- Transfusion history: no transfusions
- Pregnancy history:
- Anti-D Ig history:
- Context:
- Hemolytic consequences:
- Comment:
Antibodies in D negative recipients
Alloimmunization in recipients: expected to be possible, see phenotype data
Reports
Summary: NA, NA (last update: Aug. 25, 2020)Detailed reports
- 9/342 out of 342 D samples sent for D phenotyping (76 were too weak for epitope determination and classification, only half of the remaining could be classified) White ##### Black ##### Japanese
- 3 related samples American (unclear: Italian descent?)
- 4 samples (3 related) English (unclear: Italian descent?)
- 2 related samples Australian (unclear: Italian descent?)
- 1 sample Thailand
- 4/168 among 168 samples referred for weak D phenotype and/or allo anti-D in D positive individuals, 137 were characterized in the study (70 by serology, 67 by molecular analysis, 31 could not be typed because serologic typing was inconclusive and molecular typing could not be performed) in the French population (Caucasian)
- 0/10000 screening of D positive individuals Indian (West India)
- 1 sample alloimmunized woman Portuguese
Allele or phenotype frequency
Structure mapping
Movement | Mouse Input | Touch Input | ||
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Rotation | Primary Mouse Button | Single touch | ||
Translation | Middle Mouse Button or Ctrl+Primary | Triple touch | ||
Zoom | Scroll Wheel or Second Mouse Button or Shift+Primary | Pinch (double touch) | ||
Slab | Ctrl+Second | Not Available |
References
- Tippett P et al. The Rh antigen D: partial D antigens and associated low incidence antigens. Vox Sang, 1996. [Citation] [RHeference]
- Jones J et al. Selection of monoclonal antibodies for the identification of D variants: ability to detect weak D and to split epD2, epD5 and epD6/7. Vox Sang, 1996. [Citation] [RHeference]
- Jones JW et al. Quantitation of Rh D antigen sites on weak D and D variant red cells by flow cytometry. Vox Sang, 1996. [Citation] [RHeference]
- Cartron JP et al. Tentative model for the mapping of D epitopes on the RhD polypeptide. Transfus Clin Biol, 1996. [Citation] [RHeference]
- Avent ND et al. Evidence of genetic diversity underlying Rh D-, weak D (Du), and partial D phenotypes as determined by multiplex polymerase chain reaction analysis of the RHD gene. Blood, 1997. [Citation] [RHeference]
- Wallace M et al. DBT: a partial D phenotype associated with the low-incidence antigen Rh32. Transfus Med, 1997. [Citation] [RHeference]
- Reid ME et al. Two examples of an inseparable antibody that reacts equally well with DW+ and Rh32+ red blood cells. Vox Sang, 1998. [Citation] [RHeference]
- Reid ME et al. Use of LOR-15C9 monoclonal anti-D to differentiate erythrocytes with the partial DvI antigen from those with either partial D antigens or weak D antigens. Immunohematology, 1998. [Citation] [RHeference]
- M. J. Rodrigues et al. Identification of partial D antigen, DBT, with allo-anti-D in the serum Transfusion Medicine, 2002. — Abstract — [RHeference]
- Reid ME et al. DAK, a new low-incidence antigen in the Rh blood group system. Transfusion, 2003. [Citation] [RHeference]
- Ansart-Pirenne H et al. RhD variants in Caucasians: consequences for checking clinically relevant alleles. Transfusion, 2004. [Citation] [RHeference]
- Kulkarni S et al. Frequency of partial D in Western India. Transfus Med, 2008. [Citation] [RHeference]
- Floch A et al. Comment from Rheference Online ressource, 2020. — Online ressource — [RHeference]
Last update: June 16, 2020